Fe/starch nanoparticle - Pseudomonas aeruginosa: Bio-physiochemical and MD studies

Int J Biol Macromol. 2018 Oct 1:117:51-61. doi: 10.1016/j.ijbiomac.2018.04.191. Epub 2018 May 4.

Abstract

In this research, we attempt to study biosurfactant production by Pseudomonas aeruginosa using Fe/starch nanoparticles. Fe/starch showed no bacterial toxicity at 1 mg/ml and increased the growth rate and biosurfactant production up to 23.91 and 20.62%, respectively. Surface tension, dry weight cell, and emulsification indexes (E24) were measured. Biosurfactant production was considered via computational techniques and molecular dynamic (MD) simulation through flexible and periodic conditions (by material studio software) as well. The results of software predictions demonstrate by radial distribution function (RDF), density, energy and temperature graphs. According to the present experimental results, increased 30% growth of the bacterium has been observed and the subsequent production of biosurfactant. The difference between the experimental results and simulation data were achieved up to 0.17 g/cm3, which confirms the prediction of data by the software due to a difference of less than 14.5% (ideal error value is 20%).

Keywords: Biosurfactant; Fe/starch; MD; Microorganisms; Pseudomonas aeruginosa.

MeSH terms

  • Biomass
  • Chemical Phenomena
  • Emulsions
  • Iron / chemistry*
  • Metal Nanoparticles / chemistry*
  • Molecular Conformation
  • Molecular Dynamics Simulation
  • Molecular Structure
  • Pseudomonas aeruginosa* / growth & development
  • Spectroscopy, Fourier Transform Infrared
  • Starch / chemistry*
  • Structure-Activity Relationship
  • Surface Tension

Substances

  • Emulsions
  • Starch
  • Iron