Biological nitrogen fixation is critical for the nitrogen cycle on the earth. Nitrogen-fixing bacteria, as an environmentally friendly microorganism, convert atmospheric nitrogen to available nitrogen source for plants. In this study, we found that carbon dots (CDs) could significantly enhance the nitrogen-fixing activity of azotobacter chroococcum, in which the activity of azotobacter treated with CDs (4 μg/mL) was increased by 158% compared to that of the control one. A series of experiments suggest that CDs can combine with the nitrogenase, affect the secondary structure of nitrogenase, improve the electron transfer in the biocatalytic process, and finally improve nitrogenase activity for nitrogen fixation. Our findings may offer an economical and environmentally friendly means of improving the biological nitrogen fixation as well as solving the insufficiency of nitrogen fertilizer.
Keywords: azotobacter chroococcum; biological nitrogen fixation; carbon dots; nitrogen fixation activity; nitrogenase.