Immune Profiling of T Cells Infiltrating Vitreous Humor in Tubercular Uveitis

Immunol Invest. 2018 Aug;47(6):615-631. doi: 10.1080/08820139.2018.1470640. Epub 2018 May 7.

Abstract

Purpose: To assess cellular composition and local cytokine response in vitreous humor of tubercular uveitis.

Methods: Cells were collected from vitreous cassettes and peripheral blood of 8 tubercular uveitis and 5 control subjects, undergoing vitrectomy and analyzed by flow cytometry for cellular composition, activation status, proinflammatory cytokine expression, and uptake of TLR9 ligand, CpG ODN 2216.

Results: CD3 + T cells with equal proportion of CD4+ and CD8 + T cells formed major fraction of infiltrating cells. The vitreous humor showed higher expression of recent activation marker, CD69, and proinflammatory cytokines, IFN-γ and IL-17A, in CD4 + T cells as compared to peripheral blood. Lastly, intraocular CD4 + T cells showed reduced uptake of ODN 2216 than peripheral blood.

Conclusions: Our results indicate that local antigenic stimuli trigger T cell infiltration and activation of CD4 + T cells that are hyporesponsive to TLR9 stimulation. These infiltrating T cells might be responsible in further aggravating ocular inflammation.

Keywords: Activated T cell; T cells; TLR9; Th1 response; Th17 response; Tubercular uveitis; Vitreous humor.

MeSH terms

  • Adult
  • Aged
  • Antigens, CD / metabolism
  • Antigens, Differentiation, T-Lymphocyte / metabolism
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Female
  • Humans
  • Interferon-gamma / metabolism
  • Interleukin-17 / metabolism
  • Lectins, C-Type / metabolism
  • Lymphocyte Activation / immunology*
  • Male
  • Middle Aged
  • Oligonucleotides / metabolism
  • Toll-Like Receptor 9 / immunology
  • Tuberculosis, Ocular / immunology*
  • Tuberculosis, Ocular / microbiology
  • Uveitis / immunology*
  • Uveitis / microbiology
  • Vitreous Body / cytology*
  • Vitreous Body / immunology*
  • Vitreous Body / microbiology
  • Young Adult

Substances

  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD69 antigen
  • IFNG protein, human
  • IL17A protein, human
  • Interleukin-17
  • Lectins, C-Type
  • Oligonucleotides
  • TLR9 protein, human
  • Toll-Like Receptor 9
  • Interferon-gamma