Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling

Nat Methods. 2018 Jun;15(6):437-439. doi: 10.1038/s41592-018-0007-1. Epub 2018 May 7.


Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Associated Protein 9 / metabolism*
  • Chromatin Immunoprecipitation
  • DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism*
  • Endonucleases
  • Genome
  • Genomics
  • HEK293 Cells
  • Humans
  • Multifunctional Enzymes
  • Polymerase Chain Reaction
  • Protein Engineering


  • Multifunctional Enzymes
  • CRISPR-Associated Protein 9
  • Endonucleases
  • APEX2 protein, human
  • DNA-(Apurinic or Apyrimidinic Site) Lyase