[The Extract from Punica Granatum (Pomegranate) Leaves Promotes Apoptosis and Impairs Metastasis in Prostate Cancer Cells]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Jan;49(1):8-12.
[Article in Chinese]


Objective: To investigate the effects of pomegranate leaves extract(PLE)on proliferation,apoptosis and metastasis of prostate cancer cells.

Methods: The proliferation of TRAMP-C1,DU145,PC3 prostate cancer cells treated with different concentrations of PLE (final mass concentrations were 12.5,25,50,100, 200 μg/mL,respectively) for different time (24,48,72 h) was detected by MTT assay. Colony formation assay was performed to verify the long-term effects of PLE on the proliferation of DU145 and PC3 cells.After being treated with PLE for 48 h,Hoechst-33258 staining was used to observe the changes in the nucleus,the cell apoptotic rate was detected by flow cytometry,and wound-healing migration assay was perform to test the change of migration.

Results: In comparison with the control group,PLE in the range of 12.5-200 μg/mL had a certain inhibitory effect on the proliferation of TRAMP-C1,DU145 and PC3 cells ( P<0.05).In the range of 6.25-100 μg/mL,the number of colony formation of DU145 and PC3 was significantly reduced( P<0.01).After PLE treated for 48 h, the apoptotic features of nuclear fragmentation and the formation apoptotic body was observed in PC3. With the increase of concentration,the apoptotic rate increased gradually ( P<0.05),and the ability of cells to migrate to the scratch area was significantly weaker than the control group ( P<0.01).

Conclusion: PLE has effect on proliferation,apoptosis and metastasis of prostate cancer cells.

Keywords: Apoptosis; PC3; Pomegranate leaves extract (PLE); Prostate cancer.

MeSH terms

  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Cell Proliferation
  • Humans
  • Lythraceae / chemistry*
  • Male
  • Neoplasm Metastasis / drug therapy
  • Neoplasm Metastasis / pathology*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Prostatic Neoplasms / drug therapy
  • Prostatic Neoplasms / pathology*


  • Plant Extracts