Neutralization of Pseudomonas auruginosa Exotoxin A by human neutrophil peptide 1

Biochem Biophys Res Commun. 2018 Jun 22;501(2):454-457. doi: 10.1016/j.bbrc.2018.05.011. Epub 2018 May 10.

Abstract

Pseudomonas aeruginosa produces a large number of virulence factors, including the extracellular protein, Exotoxin A (ETA). Human Neutrophil Peptide 1 (HNP1) neutralizes the Exotoxin A. HNP1 belongs to the family of α-defensins, small effector peptides of the innate immune system that combat against microbial infections. Neutralization of bacterial toxins such as ETA by HNP1 is a novel biological function in addition to direct killing of bacteria. In this study, we report on the interaction between HNP-1 and Exotoxin A at the molecular level to allow for the design and development of potent antibacterial peptides as alternatives to classical antibiotics.

Keywords: Antimicrobial peptide; Exotoxin A; Human neutrophil peptide; Pseudomonas aeruginosa.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • ADP Ribose Transferases / metabolism*
  • ADP Ribose Transferases / toxicity*
  • Alanine / genetics
  • Amino Acid Substitution
  • Bacterial Toxins / metabolism*
  • Bacterial Toxins / toxicity*
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Exotoxins / metabolism*
  • Exotoxins / toxicity*
  • HeLa Cells
  • Humans
  • Pseudomonas aeruginosa Exotoxin A
  • Surface Plasmon Resonance
  • Virulence Factors / metabolism*
  • Virulence Factors / toxicity*
  • alpha-Defensins / administration & dosage
  • alpha-Defensins / genetics
  • alpha-Defensins / metabolism
  • alpha-Defensins / pharmacology*

Substances

  • Bacterial Toxins
  • Exotoxins
  • Virulence Factors
  • alpha-Defensins
  • human neutrophil peptide 1
  • ADP Ribose Transferases
  • Alanine