Tributyltin Exposure Induces Gut Microbiome Dysbiosis With Increased Body Weight Gain and Dyslipidemia in Mice

Environ Toxicol Pharmacol. 2018 Jun;60:202-208. doi: 10.1016/j.etap.2018.04.020. Epub 2018 May 3.

Abstract

Gut microbiome dysbiosis plays a profound role in the pathogenesis of obesity and tributyltin (TBT) has been found as an environmental obesogen. However, whether TBT could disturb gut microbiome and the relationship between obesity induced by TBT exposure and alteration in gut microbiota are still unknown. In order to assess the association between them, mice were exposed to TBTCl (50 μg kg-1) once every three days from postnatal days (PNDs) 24 to 54. The results demonstrated that TBT exposure resulted in increased body weight gain, lager visceral fat accumulation and dyslipidemia in male mice on PND 84. Correspondingly, 16S rRNA gene sequencing revealed that TBT treatment decreased gut microbial species and perturbed the microbiome composition in mice. Furthermore, Pearson's corelation coefficient analysis showed a significantly negative correlation between the body weight and the alpha diversity of gut microbiome. These results suggested that TBT exposure could induce gut microbiome dysbiosis in mice, which might contribute to the obesity pathogenesis.

Keywords: 16S ribosomal RNA gene; Dyslipidemia; Gut microbiome dysbiosis; Obesity; Tributyltin.

MeSH terms

  • Animals
  • Bacteria / classification*
  • Bacteria / drug effects
  • Bacteria / genetics
  • Body Mass Index
  • Body Weight / drug effects
  • Dysbiosis / chemically induced*
  • Dysbiosis / microbiology
  • Dyslipidemias / chemically induced*
  • Dyslipidemias / microbiology
  • Gastrointestinal Microbiome / drug effects
  • Mice
  • Obesity / chemically induced*
  • Obesity / microbiology
  • RNA, Bacterial
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, RNA
  • Trialkyltin Compounds / toxicity*

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Trialkyltin Compounds
  • tributyltin