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, 3 (5), 183-190
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Putative Periodontopathic Bacteria and Herpes Viruses Interactions in the Subgingival Plaque of Patients With Aggressive Periodontitis and Healthy Controls

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Putative Periodontopathic Bacteria and Herpes Viruses Interactions in the Subgingival Plaque of Patients With Aggressive Periodontitis and Healthy Controls

Amal Elamin et al. Clin Exp Dent Res.

Abstract

The microbial profile of aggressive periodontitis patients is considered to be complex with variations among populations in different geographical areas. The aim of this study was to assess the presences of 4 putative periodontopathic bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) and 2 periodontal herpes viruses (Epstein-Barr virus type 1 [EBV-1] and human cytomegalovirus [HMCV]) in subgingival plaque of Sudanese subjects with aggressive periodontitis and healthy controls. The study group consisted of 34 subjects, 17 aggressive periodontitis patients and 17 periodontally healthy controls (14-19 years of age). Pooled subgingival plaque samples were collected and analyzed for detection of bacteria and viruses using loop-mediated isothermal amplification. Prevalence of subgingival A. actinomycetemcomitans, HCMV, and P. gingivalis were significantly higher among aggressive periodontitis patients than periodontally healthy controls. Coinfection with A. actinomycetemcomitans, HCMV, and/or EBV-1 was restricted to the cases. Increased risk of aggressive periodontitis was the highest when A. actinomycetemcomitans was detected together with EBV-1 (OD 49.0, 95% CI [2.5, 948.7], p = .01) and HCMV (OD 39.1, 95% CI [2.0, 754.6], p = .02). In Sudanese patients, A. actinomycetemcomitans and HCMV were the most associated test pathogens with aggressive periodontitis.

Keywords: Epstein–Barr virus; aggressive periodontitis; cytomegalovirus; loop‐mediated isothermal amplification (LAMP); periodontal microbiota; subgingival plaque.

Figures

Figure 1
Figure 1
The percentage of samples testing positive for targeted periodontal pathogens and viruses in the pooled subgingival plaque of the cases and controls. EBV‐1 = Epstein–Barr virus type 1; HCMV = human cytomegalovirus; A. actinomycetemcomitans = Aggregatibacter actinomycetemcomitans; P. gingivalis = Porphyromonas gingivalis; T. forsythia = Tannerella forsythia; T. denticola = Tannerella denticola
Figure 2
Figure 2
Forest plot of the association between individual periodontal pathogens and viruses and the risk of aggressive periodontitis. EBV‐1 = Epstein–Barr virus type 1; HCMV = human cytomegalovirus; OR = odds ratio; A. actinomycetemcomitans = Aggregatibacter actinomycetemcomitans; P. gingivalis = Porphyromonas gingivalis

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