A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells

Radhika Mehta; Munaum H Qureshi; Meredith K Purchal; Sylvester M Greer; Shanzhong Gong; Chinh Ngo; Emily L Que

doi:10.1039/c8cc02034e

A new probe for detecting zinc-bound carbonic anhydrase in cell lysates and cells

Chem Commun (Camb). 2018 May 24;54(43):5442-5445. doi: 10.1039/c8cc02034e.

Authors

Radhika Mehta¹, Munaum H Qureshi, Meredith K Purchal, Sylvester M Greer, Shanzhong Gong, Chinh Ngo, Emily L Que

Affiliation

¹ Department of Chemistry, University of Texas at Austin, 105 E 24th St Stop A5300, Austin, TX 78712, USA. emilyque@cm.utexas.edu.

PMID: 29745391
DOI: 10.1039/c8cc02034e

Abstract

We report the synthesis and application of a small molecule probe for carbonic anhydrase (CA) to track holo-CA in cell lysates and live-cell models of zinc dyshomeostasis. The probe displays a 12-fold increase in fluorescence upon binding to bovine CA and also responds to human CA isoforms.

MeSH terms

Animals
Carbonic Anhydrases / analysis*
Carbonic Anhydrases / metabolism
Cattle
Erythrocytes / cytology
Erythrocytes / metabolism*
Fluorescent Dyes / chemistry*
Humans
Molecular Structure
Small Molecule Libraries / chemistry*
Zinc / analysis*
Zinc / metabolism

Substances

Fluorescent Dyes
Small Molecule Libraries
Carbonic Anhydrases
Zinc