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. 2018 May 10;8(1):7454.
doi: 10.1038/s41598-018-25582-7.

Screening for the Anti-Inflammation Quality Markers of Xiaojin Pills Based on HPLC-MS/MS Method, COX-2 Inhibition Test and Protein Interaction Network

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Free PMC article

Screening for the Anti-Inflammation Quality Markers of Xiaojin Pills Based on HPLC-MS/MS Method, COX-2 Inhibition Test and Protein Interaction Network

Xi Xiong et al. Sci Rep. .
Free PMC article

Abstract

Nowadays, breast disorders seriously affect women's health in an increasing number. In China, Xiaojin Pills are commonly used in the treatment of breast diseases. Doctors have concluded that the combined use of Xiaojin Pills with conventional therapy can significantly improve the efficacy with fewer side effects. However, the prescription of Xiaojin Pills is complicated and their quality control methods cannot completely ensure the quality of Xiaojin Pills. On the basis of its mechanism, our study combined chemical evaluation and biological evaluation to identify the anti-inflammatory markers of Xiaojin Pills. In this manuscript, 13 compounds in Xiaojin Pills were quantified. At the same time, the cyclooxygenase-2 inhibition rates of different Xiaojin Pills were measured and the possible markers were screened by spectrum-effect relationship. Further, anti-inflammatory activities of markers were verified and protein interaction network was analyzed, identifying the components of Protocatechuate, Beta-Boswellic acid and Levistilide A as the anti-inflammatory quality markers of Xiaojin Pills. We hope our studies can provide a scientific theoretical basis for accurately quality control of Xiaojin Pills and reasonable suggestions for pharmaceutical companies and new ideas for the quality control of other medicines.

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
The screening process of Xiaojin Pills’ quality markers based on multi component content determination, COX-2 anti-inflammatory experiment and network interaction analysis.
Figure 2
Figure 2
CA result of different batches of Xiaojin Pills based on chemical analysis.
Figure 3
Figure 3
Concentration-COX-2 inhibition rate- curves of different Xiaojin Pills’ samples and three compounds. S4 (A), S1 (B), S6 (C), Pro (D), Bet (E) and Lev (F).
Figure 4
Figure 4
(A) The protein interaction network of Pro, Bet and Lev; (B) the modules of the protein interaction network of Pro, Bet and Lev.
Figure 5
Figure 5
Typical MRM and chemical structures of the 13 compounds in Xiaojin Pills.

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