Rapid identification of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction coupled with gas chromatography-mass spectrometry

Food Chem. 2018 Oct 1:262:7-13. doi: 10.1016/j.foodchem.2018.04.088. Epub 2018 Apr 23.

Abstract

A novel approach for rapid identification of three foodborne pathogens including Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei in foods by solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was established. After cultivation 24, 18 and 20 h for Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei, respectively, the microbial volatile organic compounds (MVOCs) were extracted with a SPME device equipped with divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) coated fibers. The DB-1701P column was applied for separation of MVOCs. A total of 17, 13 and 14 volatile organic compounds were identified as characteristic MVOCs of Staphylococcus aureus, Vibrio parahaemolyticus and Shigella sonnei, respectively. Similarity of the MVOC chromatographic fingerprints for the bacteria were calculated and compared, and the results showed that the established method is stable, reproducible, accurate and has the potential to identify the three bacteria in food samples.

Keywords: Chromatographic fingerprint; Food; Foodborne pathogen; Gas chromatography–mass spectrometry; Microbial volatile organic compounds; Similarity match; Solid phase microextraction.

MeSH terms

  • Dimethylpolysiloxanes / chemistry
  • Food Microbiology / methods*
  • Gas Chromatography-Mass Spectrometry / methods*
  • Polyvinyls / chemistry
  • Shigella sonnei / isolation & purification
  • Solid Phase Microextraction / instrumentation
  • Solid Phase Microextraction / methods*
  • Staphylococcus aureus / isolation & purification
  • Vibrio parahaemolyticus / isolation & purification
  • Volatile Organic Compounds / analysis
  • Volatile Organic Compounds / isolation & purification

Substances

  • Dimethylpolysiloxanes
  • Polyvinyls
  • Volatile Organic Compounds
  • poly(dimethylsiloxane-divinylbenzene)