Next-generation antibodies for post-translational modifications

Curr Opin Struct Biol. 2018 Aug;51:141-148. doi: 10.1016/j.sbi.2018.04.006. Epub 2018 May 10.

Abstract

Despite increasing demands for antibodies to post-translational modifications (PTMs), fundamental difficulties in molecular recognition of PTMs hinder the generation of highly functional anti-PTM antibodies using conventional methods. Recently, advanced approaches in protein engineering and design that have been established for biologics development were applied to successfully generating highly functional anti-PTM antibodies. Furthermore, structural analyses of anti-PTM antibodies revealed unprecedented binding modes that substantially increased the antigen-binding surface. These features deepen the understanding of mechanisms underlying specific recognition of PTMs, which may lead to more effective approaches for generating anti-PTM antibodies with exquisite specificity and high affinity.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Antibodies / chemistry*
  • Antibodies / metabolism
  • Antibodies / pharmacology*
  • Drug Design
  • Models, Molecular
  • Protein Binding
  • Protein Conformation
  • Protein Engineering*
  • Protein Processing, Post-Translational / drug effects*
  • Structure-Activity Relationship

Substances

  • Antibodies