Blood-derived integration-free iPS cell line UKBi011-A from a diagnosed male Alzheimer's disease patient with APOE ɛ4/ɛ4 genotype

Stem Cell Res. 2018 May;29:250-253. doi: 10.1016/j.scr.2018.04.011. Epub 2018 Apr 23.

Abstract

Alzheimer's disease (AD) is most the frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for late-onset AD. Here, we present an iPSC line generated from peripheral blood cells of a male AD patient employing Sendai virus vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The characterized iPSC line expresses typical human pluripotency markers and shows differentiation into all three germ layers, complete reprogramming vector clearance, a normal SNP genotype and maintenance of the APOE ε4/ε4 allele.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged, 80 and over
  • Alzheimer Disease* / diagnosis
  • Alzheimer Disease* / genetics
  • Alzheimer Disease* / metabolism
  • Alzheimer Disease* / pathology
  • Apolipoprotein E4* / genetics
  • Apolipoprotein E4* / metabolism
  • Blood Cells* / metabolism
  • Blood Cells* / pathology
  • Cellular Reprogramming Techniques
  • Genotype*
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Induced Pluripotent Stem Cells* / pathology
  • Male
  • Polymorphism, Single Nucleotide*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics

Substances

  • Apolipoprotein E4
  • Transcription Factors