Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

Gamal Wareth; Mohamed El-Diasty; Falk Melzer; Jayaseelan Murugaiyan; Amir Abdulmawjood; Lisa D Sprague; Heinrich Neubauer

doi:10.1155/2018/2056436

Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

Vet Med Int. 2018 Mar 20:2018:2056436. doi: 10.1155/2018/2056436. eCollection 2018.

Authors

Gamal Wareth^{1

2}, Mohamed El-Diasty³, Falk Melzer¹, Jayaseelan Murugaiyan⁴, Amir Abdulmawjood⁵, Lisa D Sprague¹, Heinrich Neubauer¹

Affiliations

¹ Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
² Department of Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt.
³ Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt.
⁴ Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany.
⁵ Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany.

Abstract

Trueperella pyogenes was isolated from a dog and a cat with a mixed infection with Brucella abortus. Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. Identification of the bacteria was done by means of MALDI-TOF MS, loop-mediated isothermal amplification (LAMP) based on cpn60, partial 16S rRNA sequencing, and growth on Loeffler Serum Medium. Isolation of Trueperella pyogenes on the dairy farm highlights its neglected role in reproduction failure and draws attention to its effects in the dairy industry in Egypt. Diagnosis and control of abortion in Egypt should include Trueperella pyogenes as one of possible causes of abortion.