SOX7 Target Genes and Their Contribution to Its Tumor Suppressive Function

Int J Mol Sci. 2018 May 14;19(5):1451. doi: 10.3390/ijms19051451.

Abstract

SOX7 is a transcription factor and acts as a tumor suppressor, but its target genes in cancers are poorly explored. We revealed SOX7-mediated gene expression profile in breast cancer cells using microarray chips and discovered multiple altered signaling pathways. When combinatorially analyzing the microarray data with a gene array dataset from 759 breast cancer patients, we identified four genes as potential targets of SOX7 and validated them by quantitative PCR and chromatin immunoprecipitation assays. Among these four genes, we determined that SOX7-activated SPRY1 and SLIT2, and SOX7-repressed TRIB3 and MTHFD2 could all differentially contribute to SOX7-mediated tumor suppression. Overall, we identified multiple cancer-related pathways mediated by SOX7 and for the first time revealed SOX7-regulated target genes in a cancer-relevant context.

Keywords: SOX7; breast cancer; cell proliferation; gene expression array; target genes.

MeSH terms

  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Cell Line, Tumor
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Genes, Reporter
  • Humans
  • Protein Binding
  • Reproducibility of Results
  • SOXF Transcription Factors / metabolism*
  • Transcriptome
  • Tumor Suppressor Proteins / metabolism*

Substances

  • SOX7 protein, human
  • SOXF Transcription Factors
  • Tumor Suppressor Proteins