Humanised recombinant antibody fragments bind human pancreatic islet cells

J Immunol Methods. 2018 Aug;459:20-28. doi: 10.1016/j.jim.2018.05.006. Epub 2018 May 21.

Abstract

We describe here the humanisation of two mouse monoclonal antibodies that bind to surface markers on human pancreatic islet endocrine cells. Monoclonal antibodies produced by the HIC1-2B4 and HIC0-4F9 mouse hybridomas bind distinct surface molecules expressed on endocrine cells and have been validated for a number of experimental methods including immunohistochemistry and live cell sorting by flow cytometry. Variable region framework and first constant region domain sequences were replaced with that from compatible human antibody sequences, and the resulting recombinant antigen-binding fragments were cloned and expressed in mouse myeloma cells. ELISA, fluorescent immunohistochemistry, and flow cytometry were used to assess the specificity of the humanised antibody fragments. Purification and binding analyses indicated that human islet endocrine cell binding was retained in the humanised antibody fragments. These humanised, recombinant antibody fragments have a lower risk of eliciting adverse responses from a patient's immune system and, therefore, have highly improved clinical potential. Thus, they may be used to image, target or carry cargo specifically to islet cells in human patients.

Keywords: Beta cells; Humanisation; Islets; Monoclonal antibody; Recombinant antibody, antigen-binding fragment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal, Humanized / isolation & purification
  • Antibodies, Monoclonal, Humanized / metabolism*
  • Antibody Specificity
  • Binding Sites, Antibody
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Humans
  • Hybridomas
  • Immunoglobulin Fab Fragments / isolation & purification
  • Immunoglobulin Fab Fragments / metabolism*
  • Immunohistochemistry
  • Islets of Langerhans / immunology*
  • Islets of Langerhans / metabolism*
  • Mice
  • Models, Molecular
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism

Substances

  • Antibodies, Monoclonal, Humanized
  • Immunoglobulin Fab Fragments
  • Recombinant Proteins