CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

Nucleic Acids Res. 2018 Jul 2;46(W1):W242-W245. doi: 10.1093/nar/gky354.

Abstract is a web tool for genome editing experiments with the CRISPR-Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from, including the full source code of the website and a stand-alone, command-line version.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • CRISPR-Associated Protein 9
  • CRISPR-Cas Systems*
  • Gene Editing*
  • Gene Knockout Techniques
  • Genome
  • Internet
  • Mutagenesis
  • Oligonucleotides
  • RNA / chemistry*
  • Software*
  • Workflow


  • Oligonucleotides
  • RNA
  • CRISPR-Associated Protein 9