Reprogramming of Chromatin Accessibility in Somatic Cell Nuclear Transfer Is DNA Replication Independent

Cell Rep. 2018 May 15;23(7):1939-1947. doi: 10.1016/j.celrep.2018.04.036.


Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNase I hypersensitive sites (DHSs) in donor cumulus cells and one-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape of the donor cells is drastically changed to recapitulate that of the in vitro fertilization (IVF)-derived zygotes within 12 hr. Interestingly, this DHS reprogramming takes place even in the presence of a DNA replication inhibitor, suggesting that SCNT-mediated DHS reprogramming is independent of DNA replication. Thus, this study not only reveals the rapid and drastic nature of the changes in chromatin accessibility through SCNT but also establishes a DNA replication-independent model for studying cellular reprogramming.

Keywords: DNA replication; DNase I hypersensitive sites; SCNT; reprogramming.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chromatin / metabolism*
  • DNA Replication*
  • Deoxyribonuclease I / metabolism
  • Down-Regulation
  • Female
  • Mice
  • Nuclear Transfer Techniques*
  • Transcription Factors / metabolism
  • Transcription, Genetic


  • Chromatin
  • Transcription Factors
  • Deoxyribonuclease I