Structural basis for gating pore current in periodic paralysis

Nature. 2018 May;557(7706):590-594. doi: 10.1038/s41586-018-0120-4. Epub 2018 May 16.


Potassium-sensitive hypokalaemic and normokalaemic periodic paralysis are inherited skeletal muscle diseases characterized by episodes of flaccid muscle weakness1,2. They are caused by single mutations in positively charged residues ('gating charges') in the S4 transmembrane segment of the voltage sensor of the voltage-gated sodium channel Nav1.4 or the calcium channel Cav1.11,2. Mutations of the outermost gating charges (R1 and R2) cause hypokalaemic periodic paralysis1,2 by creating a pathogenic gating pore in the voltage sensor through which cations leak in the resting state3,4. Mutations of the third gating charge (R3) cause normokalaemic periodic paralysis 5 owing to cation leak in both activated and inactivated states 6 . Here we present high-resolution structures of the model bacterial sodium channel NavAb with the analogous gating-charge mutations7,8, which have similar functional effects as in the human channels. The R2G and R3G mutations have no effect on the backbone structures of the voltage sensor, but they create an aqueous cavity near the hydrophobic constriction site that controls gating charge movement through the voltage sensor. The R3G mutation extends the extracellular aqueous cleft through the entire length of the activated voltage sensor, creating an aqueous path through the membrane. Conversely, molecular modelling shows that the R2G mutation creates a continuous aqueous path through the membrane only in the resting state. Crystal structures of NavAb(R2G) in complex with guanidinium define a potential drug target site. Molecular dynamics simulations illustrate the mechanism of Na+ permeation through the mutant gating pore in concert with conformational fluctuations of the gating charge R4. Our results reveal pathogenic mechanisms of periodic paralysis at the atomic level and suggest designs of drugs that may prevent ionic leak and provide symptomatic relief from hypokalaemic and normokalaemic periodic paralysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Electric Conductivity
  • Guanidine / metabolism
  • Humans
  • Hypokalemic Periodic Paralysis / genetics
  • Hypokalemic Periodic Paralysis / metabolism
  • Ion Channel Gating* / genetics
  • Molecular Dynamics Simulation
  • Mutation
  • NAV1.4 Voltage-Gated Sodium Channel / chemistry*
  • NAV1.4 Voltage-Gated Sodium Channel / genetics
  • NAV1.4 Voltage-Gated Sodium Channel / metabolism*
  • Paralyses, Familial Periodic / genetics
  • Paralyses, Familial Periodic / metabolism*
  • Sodium / metabolism
  • Thermodynamics


  • NAV1.4 Voltage-Gated Sodium Channel
  • SCN4A protein, human
  • Sodium
  • Guanidine