Membrane association of monotopic phosphoglycosyl transferase underpins function

Leah C Ray; Debasis Das; Sonya Entova; Vinita Lukose; Andrew J Lynch; Barbara Imperiali; Karen N Allen

doi:10.1038/s41589-018-0054-z

Membrane association of monotopic phosphoglycosyl transferase underpins function

Nat Chem Biol. 2018 Jun;14(6):538-541. doi: 10.1038/s41589-018-0054-z. Epub 2018 May 16.

Authors

Leah C Ray¹, Debasis Das^{2

3}, Sonya Entova², Vinita Lukose³, Andrew J Lynch⁴, Barbara Imperiali^{5

6}, Karen N Allen^{7

8}

Affiliations

¹ Program in Biomolecular Pharmacology, Boston University School of Medicine, Boston, MA, USA.
² Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
³ Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
⁴ Department of Chemistry, Boston University, Boston, MA, USA.
⁵ Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. imper@mit.edu.
⁶ Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA. imper@mit.edu.
⁷ Program in Biomolecular Pharmacology, Boston University School of Medicine, Boston, MA, USA. drkallen@bu.edu.
⁸ Department of Chemistry, Boston University, Boston, MA, USA. drkallen@bu.edu.

Abstract

Polyprenol phosphate phosphoglycosyl transferases (PGTs) catalyze the first membrane-committed step in assembly of essential glycoconjugates. Currently there is no structure-function information to describe how monotopic PGTs coordinate the reaction between membrane-embedded and soluble substrates. We describe the structure and mode of membrane association of PglC, a PGT from Campylobacter concisus. The structure reveals a unique architecture, provides mechanistic insight and identifies ligand-binding determinants for PglC and the monotopic PGT superfamily.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Campylobacter / enzymology*
Catalysis
Catalytic Domain
Cell Membrane / enzymology*
Cloning, Molecular
Cysteine / chemistry
Glycoconjugates / chemistry
Glycosyltransferases / chemistry*
Ligands
Mutation
Phosphates / chemistry*
Phosphorylation
Protein Domains
Protein Folding
Protein Structure, Secondary
Structure-Activity Relationship
Substrate Specificity

Substances

Glycoconjugates
Ligands
Phosphates
Glycosyltransferases
Cysteine

Abstract

Publication types

MeSH terms

Substances

Grants and funding