3D bioprinting of a corneal stroma equivalent

Exp Eye Res. 2018 Aug;173:188-193. doi: 10.1016/j.exer.2018.05.010. Epub 2018 May 30.


Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered.

Keywords: 3D bioprinting; Bio-ink; Collagen; Cornea; Keratocytes; Tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioartificial Organs
  • Bioprinting / methods*
  • Corneal Keratocytes / cytology*
  • Corneal Stroma / cytology*
  • Equipment Design
  • Humans
  • Printing, Three-Dimensional / instrumentation*
  • Tissue Engineering
  • Tissue Scaffolds