IL-33/s-ST2 ratio, systemic symptoms, and basophil activation in Pru p 3-sensitized allergic patients

Inflamm Res. 2018 Aug;67(8):671-679. doi: 10.1007/s00011-018-1157-4. Epub 2018 May 17.


Background: Although IL-33/ST2 axis is involved in the development of allergic diseases, its contribution in food allergy is still unknown.

Methods: In this study, we assessed the serum levels of IL-33 and its s-ST2 receptor in 53 control patients (without allergic diseases), 47 peach (Pru p 3)-sensitized allergic patients (SAP), and in 68 non-Pru p 3-SAP. Basophil activation test (BAT) was used to assess the basophil activation due to allergen exposure before and after the addition of s-ST2 to the blood samples from 5 Pru p 3-SAP.

Results: IL-33 levels in Pru p 3-SAP were higher than in non-Pru p 3-SAP and in normal controls. Lower s-ST2 levels were found in Pru p 3-SAP than in non-Pru p 3-SAP. IL-33/s-ST2 ratio was higher in Pru p 3-SAP than in both non-Pru p 3-SAP and controls. Higher IL-33/s-ST2 ratio was observed in Pru p 3-SAP with severe than in those with mild systemic symptoms. BAT analysis in Pru p 3-SAP showed a decrease in basophil activation due to Pru p 3 exposure after the addition of s-ST2 to the blood samples.

Conclusions: An imbalance in the baseline levels of IL-33/ST2 pathway is present in Pru p 3-SAP. The measurement of this pathway might be helpful to detect patients at a higher risk of developing severe systemic symptoms.

Keywords: Anaphylaxis; Basophil activation test; Food allergy; IL-33 receptor; Non-specific lipid-transfer protein; Panallergens; Peach allergy; sIgE to Pru p 3; sST2.

MeSH terms

  • Adolescent
  • Adult
  • Allergens / immunology*
  • Antigens, Plant / immunology*
  • Asthma / blood
  • Asthma / immunology
  • Basophils / immunology
  • Calcifediol / blood
  • Female
  • Food Hypersensitivity / blood*
  • Food Hypersensitivity / immunology
  • Humans
  • Immunoglobulin E / blood
  • Interleukin-1 Receptor-Like 1 Protein / blood*
  • Interleukin-1 Receptor-Like 1 Protein / immunology
  • Interleukin-33 / blood*
  • Interleukin-33 / immunology
  • Male
  • Middle Aged
  • Plant Proteins / immunology*
  • Rhinitis / blood
  • Rhinitis / immunology
  • Young Adult


  • Allergens
  • Antigens, Plant
  • IL1RL1 protein, human
  • IL33 protein, human
  • Interleukin-1 Receptor-Like 1 Protein
  • Interleukin-33
  • Plant Proteins
  • Pru p 3 allergen
  • Immunoglobulin E
  • Calcifediol