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. 2018 Sep;92(1):78-83.
doi: 10.1016/j.diagmicrobio.2018.04.003. Epub 2018 Apr 14.

Simultaneous Quantification of Systemic Azoles and Their Major Metabolites in Human Serum by HPLC/PDA: Role of Azole Metabolic Rate

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Simultaneous Quantification of Systemic Azoles and Their Major Metabolites in Human Serum by HPLC/PDA: Role of Azole Metabolic Rate

Alicia Gomez-Lopez et al. Diagn Microbiol Infect Dis. .

Abstract

We describe the development and validation of a novel liquid chromatography assay (HPLC/PDA) for simultaneous quantification of voriconazole, itraconazole, and posaconazole, as well as some of their major metabolites, voriconazole N-oxide and hydroxy-itraconazole, in human serum. Analytes were detected using a PDA system that allows the characterization of the specific UV spectra of each compound. The assay exhibited linearity between 0.25-16 mg/L for all the compounds tested. The accuracy and within- and between-day precision of the assay were in acceptable ranges. We successfully quantified the azoles and some of their metabolites in a collection of clinical samples collected from treated patients. The method also allows assessing the metabolic rate of several azoles being useful to predict the metabolic profile of a particular patient and to anticipate toxicity or efficacy during the treatment.

Keywords: HPLC; azole drugs; hydroxy itraconazole; metabolic rate; therapeutic drug monitoring; voriconazole N-oxide.

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