Comparsion of staining characteristics of Toto bodies

Biotech Histochem. 2018;93(5):336-339. doi: 10.1080/10520295.2018.1441441. Epub 2018 May 21.


Toto bodies are eosinophilic structures that resemble the cells of the superficial cell layer of the oral epithelium. Toto bodies commonly are associated with inflammatory gingival and other mucosal lesions including pyogenic granuloma, irritational fibroma, epulis fissuratum, peripheral giant cell granuloma and inflammatory hyperplastic gingivitis. We evaluated staining characteristics of Toto bodies to establish their origin and to identify their significance in lesions. We investigated pyogenic granuloma, fibroma and leukoplakia with epithelium that exhibited Toto bodies after hematoxylin and eosin (staining. Sections were stained with Alcian blue, periodic acid-Schiff and Ayoub-Shklar stains to evaluate staining intensity and distribution. More Toto bodies were found in pyogenic granuloma than in fibroma and leukoplakia. PAS and Alcian blue staining exhibited mild intensity and did not establish the origin of Toto bodies. High staining intensity and diffuse distribution of stain was observed using Ayoub-Shklar staining, which indicated that Toto bodies originate from keratin.

Keywords: Alcian blue; Ayoub-Shklar; Toto bodies; keratin; mucopolysaccharide keratin dystrophy; periodic acid-Schiff.

Publication types

  • Comparative Study

MeSH terms

  • Alcian Blue
  • Eosine Yellowish-(YS)
  • Epithelial Cells
  • Epithelium
  • Fibroma
  • Hematoxylin
  • Humans
  • Keratins / metabolism
  • Mouth Mucosa / cytology
  • Staining and Labeling*


  • Keratins
  • Alcian Blue
  • Eosine Yellowish-(YS)
  • Hematoxylin