SPR Biosensors in Direct Molecular Fishing: Implications for Protein Interactomics

Sensors (Basel). 2018 May 18;18(5):1616. doi: 10.3390/s18051616.


We have developed an original experimental approach based on the use of surface plasmon resonance (SPR) biosensors, applicable for investigation of potential partners involved in protein⁻protein interactions (PPI) as well as protein⁻peptide or protein⁻small molecule interactions. It is based on combining a SPR biosensor, size exclusion chromatography (SEC), mass spectrometric identification of proteins (LC-MS/MS) and direct molecular fishing employing principles of affinity chromatography for isolation of potential partner proteins from the total lysate of biological samples using immobilized target proteins (or small non-peptide compounds) as ligands. Applicability of this approach has been demonstrated within the frame of the Human Proteome Project (HPP) and PPI regulation by a small non-peptide biologically active compound, isatin.

Keywords: LC-MS/MS protein identification; SPR biosensors; direct molecular fishing; isatin; protein interactomics; size exclusion chromatography (SEC).

Publication types

  • Review

MeSH terms

  • Biosensing Techniques*
  • Chromatography, Gel
  • Immobilized Proteins / chemistry
  • Ligands
  • Mass Spectrometry
  • Protein Binding
  • Protein Interaction Maps*
  • Proteins / chemistry*
  • Small Molecule Libraries / chemistry
  • Surface Plasmon Resonance / methods*
  • Tandem Mass Spectrometry


  • Immobilized Proteins
  • Ligands
  • Proteins
  • Small Molecule Libraries