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. 2018 Jul;36(6):509-511.
doi: 10.1038/nbt.4136. Epub 2018 May 14.

Reversal of siRNA-mediated Gene Silencing in Vivo

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Reversal of siRNA-mediated Gene Silencing in Vivo

Ivan Zlatev et al. Nat Biotechnol. .

Abstract

We report rapid, potent reversal of GalNAc-siRNA-mediated RNA interference (RNAi) activity in vivo with short, synthetic, high-affinity oligonucleotides complementary to the siRNA guide strand. We found that 9-mers with five locked nucleic acids (LNAs) have the highest potency across several targets. Our modular, sequence-specific approach, named REVERSIR, may enhance the therapeutic profile of any long-acting GalNAc-siRNA (short interfering RNA) conjugate by enabling control of RNAi pharmacology.

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References

    1. N Engl J Med. 2013 Aug 29;369(9):819-29 - PubMed
    1. Nucleic Acids Res. 2005 Nov 27;33(20):e179 - PubMed
    1. Science. 2014 Oct 31;346(6209):608-13 - PubMed
    1. Nat Commun. 2018 Feb 19;9(1):723 - PubMed
    1. RNA. 2010 Dec;16(12):2553-63 - PubMed

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