Reproducibility of 2D GluCEST in healthy human volunteers at 7 T

Magn Reson Med. 2018 Nov;80(5):2033-2039. doi: 10.1002/mrm.27362. Epub 2018 May 25.


Purpose: To investigate the reproducibility of gray and white matter glutamate contrast of a brain slice among a small group of healthy volunteers by using the 2D single-slice glutamate CEST (GluCEST) imaging technique.

Methods: Six healthy volunteers were scanned multiple times for within-day and between-day reproducibility. One more volunteer was scanned for within-day reproducibility at 7T MRI. Glutamate CEST contrast measurements were calculated for within subjects and among the subjects and the coefficient of variations are reported.

Results: The GluCEST measurements were highly reproducible in the gray and white matter area of the brain slice, whether it was within-day or between-day with a coefficient of variation of less than 5%.

Conclusion: This preliminary study in a small group of healthy volunteers shows a high degree of reproducibility of GluCEST MRI in brain and holds promise for implementation in studying age-dependent changes in the brain.

Keywords: GluCEST; GluCEST reproducibility; glutamate.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Aged
  • Brain / diagnostic imaging*
  • Brain / metabolism
  • Female
  • Glutamic Acid / chemistry*
  • Glutamic Acid / metabolism
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Magnetic Resonance Imaging / methods*
  • Magnetic Resonance Imaging / standards*
  • Male
  • Middle Aged
  • Reproducibility of Results


  • Glutamic Acid