microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2

Arch Biochem Biophys. 2018 Aug 1;651:52-60. doi: 10.1016/j.abb.2018.05.018. Epub 2018 May 23.

Abstract

Adriamycin resistance is closely related to therapeutic efficacy in breast cancer patients and their prognosis. Increasing evidence has suggested that miRNA functions in Adriamycin resistance in various types of cancer. microRNA-129-5p (miR-129-5p) has been considered a tumor-suppressive miRNA in several cancers, but its potential role in Adriamycin resistance in breast cancer has not been fully elucidate. By qRT-PCR assay, we revealed that the expression of miR-129-5p was significantly decreased in breast cancer tissues and Adriamycin-resistant breast cancer cells (MDA-MB-231/ADR, MCF-7/ADR). CCK-8, colony formation, wound healing, Transwell invasion, and flow cytometric profiles were examined to determine the influence of miR-129-5p on Adriamycin-resistant breast cancer in vitro. The upregulation of miR-129-5p decreased the IC50 concentration of Adriamycin and invasion and promoted the apoptosis of MDA-MB-231/ADR cells in the presence of Adriamycin, whereas the upregulation of Sex-Determining Region Y-Box 2 (SOX2) reversed these effects. A luciferase reporter assay confirmed the binding of miR-129-5p to the 3'UTR of SOX2. Collectively, it was suggested that miR-129-5p suppresses Adriamycin resistance in breast cancer by directly targeting SOX2.

Keywords: Adriamycin resistance; Breast cancer; SOX2; miR-129-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antineoplastic / pharmacology*
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / genetics
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Doxorubicin / pharmacology*
  • Drug Resistance, Neoplasm*
  • Female
  • Gene Expression Regulation, Neoplastic* / drug effects
  • Humans
  • MCF-7 Cells
  • MicroRNAs / genetics*
  • SOXB1 Transcription Factors / genetics*

Substances

  • Antibiotics, Antineoplastic
  • MicroRNAs
  • Mirn129 microRNA, human
  • SOX2 protein, human
  • SOXB1 Transcription Factors
  • Doxorubicin