Identification of Campylobacter fetus by fluorescence in situ hybridization (FISH)

J Microbiol Methods. 2018 Aug;151:44-47. doi: 10.1016/j.mimet.2018.05.020. Epub 2018 May 24.

Abstract

Two new DNA FISH-probes for Campylobacter fetus were designed, in silico checked for cross-reactions and successfully evaluated in a multi-centric approach with 41 Campylobacter fetus isolates including isolates of all three know subspecies: Campylobacter fetus ssp. fetus, Campylobacter fetus ssp. venerealis, and Campylobacter fetus ssp. testudinum and 40 strains of five non-target Campylobacter species.

Keywords: Bacteremia; Campylobacter fetus; Diagnostic microbiology; FISH; Fluorescence in situ hybridization; Genital campylobacteriosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / diagnosis
  • Bacteremia / microbiology
  • Campylobacter Infections / diagnosis*
  • Campylobacter Infections / microbiology
  • Campylobacter fetus / classification*
  • Campylobacter fetus / genetics
  • Campylobacter fetus / isolation & purification*
  • Campylobacter fetus / pathogenicity
  • DNA Probes
  • DNA, Bacterial
  • In Situ Hybridization, Fluorescence / methods*
  • Species Specificity

Substances

  • DNA Probes
  • DNA, Bacterial