Functional homology of mammalian and yeast RAS genes

Cell. 1985 Jan;40(1):19-26. doi: 10.1016/0092-8674(85)90304-6.


Yeast spores lacking endogenous RAS genes will not germinate. If such spores contain chimeric mammalian/yeast RAS genes or even the mammalian H-ras gene under the control of the galactose-inducible GAL10 promoter, they will germinate in the presence of galactose and produce viable haploid progeny dependent on galactose for continued growth and viability. These results indicate that the biochemical function of RAS proteins is essential for vegetative haploid yeast and that this function has been conserved in evolution since the progenitors of yeast and mammals diverged.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA Restriction Enzymes
  • Fungal Proteins / genetics*
  • Genes
  • Genes, Fungal*
  • Genotype
  • Humans
  • Oncogenes*
  • Plasmids
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Species Specificity
  • Spores, Fungal / physiology
  • ras Proteins*


  • Fungal Proteins
  • DNA Restriction Enzymes
  • ras Proteins