Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor

Immunology. 1985 Feb;54(2):333-41.

Abstract

A mouse monoclonal IgG2a antibody, designated MRC OX-26, is shown to be specific for the rat transferrin receptor, but does not block transferrin binding. The antibody labelled a myeloma, three leukaemia cell lines and normal dividing cells of various types, but also bound to a number of nondividing normal tissues. No labelling of lymphopoietic stem cells could be detected, even though approximately 25% of bone marrow and over 95% of fetal liver cells were clearly labelled.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens / analysis
  • Binding Sites, Antibody
  • Bone Marrow Cells
  • Cell Separation
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Female
  • Hematopoietic Stem Cells / immunology*
  • Liver / cytology
  • Male
  • Rats
  • Rats, Inbred Strains
  • Receptors, Cell Surface / immunology*
  • Receptors, Transferrin
  • Transferrin / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Receptors, Cell Surface
  • Receptors, Transferrin
  • Transferrin