Synthesis of transcobalamin II by cultured human hepatocytes

C A Hall; P D Green-Colligan; J A Begley

doi:10.1016/0304-4165(85)90239-9

Synthesis of transcobalamin II by cultured human hepatocytes

Biochim Biophys Acta. 1985 Mar 8;838(3):387-9. doi: 10.1016/0304-4165(85)90239-9.

Authors

C A Hall, P D Green-Colligan, J A Begley

PMID: 2982421
DOI: 10.1016/0304-4165(85)90239-9

Abstract

Cultured HepG2 cells, derived from a human hepatoma synthesized and released unsaturated, immunoreactive transcobalamin II. Synthesis was confirmed by the blocking with inhibitors of protein synthesis and by incorporation of tritiated leucine into transcobalamin II.

MeSH terms

Apoproteins / biosynthesis
Carcinoma, Hepatocellular / metabolism*
Cell Line
Cycloheximide / pharmacology
Humans
Immunologic Techniques
Leucine / metabolism
Liver Neoplasms / metabolism*
Transcobalamins / biosynthesis*

Substances

Apoproteins
Transcobalamins
Cycloheximide
Leucine