Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

Cell. 1985 Mar;40(3):697-703. doi: 10.1016/0092-8674(85)90218-1.


Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acrosome / physiology*
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / physiology*
  • Calcium / metabolism
  • Fluorescent Antibody Technique
  • Glycoproteins / immunology
  • Glycoproteins / physiology*
  • Immunoglobulin Fab Fragments / immunology
  • Immunoglobulin G / immunology
  • Male
  • Membrane Proteins / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Protons
  • Sea Urchins
  • Spermatozoa / immunology
  • Spermatozoa / physiology*


  • Antibodies, Monoclonal
  • Glycoproteins
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • Membrane Proteins
  • Protons
  • Calcium