Alkaline phosphatase promotes radioprotection and accumulation of WR-1065 in V79-171 cells incubated in medium containing WR-2721

Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Jan;47(1):23-7. doi: 10.1080/09553008514550041.

Abstract

Addition of alkaline phosphatase and WR-2721 to culture medium containing V79-171 cells leads to production of WR-1065 and its disulphide forms in the medium, to cellular accumulation of WR-1065, and to radioprotection which correlates with cellular WR-1065 level.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkaline Phosphatase / pharmacology*
  • Amifostine / metabolism*
  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Cell Survival / radiation effects*
  • Cesium Radioisotopes
  • Cricetinae
  • Cricetulus
  • Gamma Rays
  • In Vitro Techniques
  • Mercaptoethylamines / metabolism*
  • Mercaptoethylamines / pharmacology
  • Organothiophosphorus Compounds / metabolism*
  • Radiation-Protective Agents / pharmacology*

Substances

  • Cesium Radioisotopes
  • Mercaptoethylamines
  • Organothiophosphorus Compounds
  • Radiation-Protective Agents
  • WR 1065
  • Alkaline Phosphatase
  • Amifostine