Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells

Nature. 1985 Feb 28-Mar 6;313(6005):812-5. doi: 10.1038/313812a0.


Epstein-Barr virus (EBV) infects human B lymphocytes, transforming the infected cells into dividing blasts that can proliferate indefinitely. The viral genome of 172 kilobase pairs (kbp) is a plasmid in most transformed cells. We have identified a region of EBV DNA, termed oriP (nucleotides 7,333-9,109 of strain B95-8), which acts in cis to permit linked DNAs to replicate as plasmids in cells containing EBV DNA. We have postulated the existence of a trans-acting gene allowing oriP function. Here we report that this gene lies in a 2.6-kbp region of the viral genome (nucleotides 107, 567-110, 176) which encodes the EBNA-1 antigen. We show that circular DNAs containing oriP, the EBNA-1 gene and a selectable marker replicate autonomously in cells derived from at least four developmental lineages and from at least three species. We also find that the one-third of the EBNA-1 gene repetitive in sequence is not essential for the trans-acting function that EBNA-1 gives oriP.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Viral / genetics
  • Cell Transformation, Viral
  • Cloning, Molecular / methods
  • Cricetinae
  • DNA Replication*
  • DNA, Viral / genetics
  • Dogs
  • Epstein-Barr Virus Nuclear Antigens
  • Gene Expression Regulation
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Mice
  • Plasmids*
  • Replicon
  • Species Specificity
  • Virus Replication*


  • Antigens, Viral
  • DNA, Viral
  • Epstein-Barr Virus Nuclear Antigens