eIF4A2 is a host factor required for efficient HIV-1 replication

Microbes Infect. Jun-Jul 2018;20(6):346-352. doi: 10.1016/j.micinf.2018.05.001. Epub 2018 May 26.

Abstract

Host factors are required for efficient HIV-1 replication. To identify these factors, genome-wide RNA interference screening was performed using a human T cell line. In the present study, we assessed whether eukaryotic translation initiation factor 4A isoform 2 (eIF4A2), a DEAD-box protein identified in our screen, is necessary for efficient HIV-1 replication. Exploiting MT4C5 cells depleted of eIF4A2 by stable expression of eIF4A2-specific short-hairpin RNA (shRNA) using a lentiviral system, we found that depletion of eIF4A2 markedly inhibited the infection of a replication-competent reporter HIV-1. eIF4A2 depletion reduced the efficiency of viral cDNA synthesis with virion entry into target cells being unaffected. Depletion of eIF4A2 also inhibited HIV-1 spreading infection in a knockdown level-dependent manner. These results suggest that HIV-1 requires eIF4A2 for optimal replication in human T cells.

Keywords: DEAD-box protein; HIV-1; Host factor; RNAi; Viral cDNA synthesis; eIF4A2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / enzymology
  • CD4-Positive T-Lymphocytes / virology
  • Cell Line
  • DNA, Viral / biosynthesis
  • Eukaryotic Initiation Factor-4A / chemistry
  • Eukaryotic Initiation Factor-4A / deficiency
  • Eukaryotic Initiation Factor-4A / genetics
  • Eukaryotic Initiation Factor-4A / metabolism*
  • Gene Silencing
  • HIV Infections / enzymology*
  • HIV Infections / virology
  • HIV-1 / physiology*
  • Host-Pathogen Interactions*
  • Humans
  • RNA, Small Interfering / genetics
  • Virus Replication*

Substances

  • DNA, Viral
  • RNA, Small Interfering
  • Eukaryotic Initiation Factor-4A