A unique type of cyclic AMP-binding protein of Trypanosoma cruzi

Mol Biochem Parasitol. 1985 Jan;14(1):75-81. doi: 10.1016/0166-6851(85)90107-0.

Abstract

On centrifugation on sucrose density gradients, the cyclic AMP-receptor protein of Trypanosoma cruzi was clearly resolved from the type II regulatory subunit of protein kinase from bovine heart (S20,W = 8.25 and 4.1, respectively). The binding of cyclic [3H]AMP to these two proteins was affected to different extents by several cyclic AMP analogues. Such differences between the cyclic AMP-receptor protein of T. cruzi and cyclic AMP-binding proteins of other eukaryotes might be exploitable by chemotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding, Competitive
  • Centrifugation, Density Gradient
  • Cyclic AMP / metabolism
  • Receptors, Cyclic AMP / analysis*
  • Trypanosoma cruzi / analysis*

Substances

  • Receptors, Cyclic AMP
  • Cyclic AMP