Multi-Parametric Screening in Arabidopsis thaliana Seedlings

Methods Mol Biol. 2018;1795:1-7. doi: 10.1007/978-1-4939-7874-8_1.


Phenotypic screening and subsequent target identification approaches are very valuable to identify chemical probes that can be used to explore the connection between phenotypes and biological pathways. However, assessing a phenotypic effect in plants in a high-throughput fashion is a challenging task and often requires expensive readout devices. In this chapter, we describe a cost-effective multi-parametric screening procedure that is compatible with liquid-handling systems and that enables the assessment of phenotypes in Arabidopsis thaliana seedlings in an automated way.

Keywords: Arabidopsis thaliana; Automation; High-content screening; Multi-parametric analysis.

MeSH terms

  • Arabidopsis / physiology*
  • Biomarkers
  • Germination
  • High-Throughput Screening Assays
  • Phenotype
  • Plants, Genetically Modified
  • Seedlings / physiology*
  • Seeds


  • Biomarkers