Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 May 29;23(9):2595-2605.
doi: 10.1016/j.celrep.2018.04.117.

Antigenic Variation in the Lyme Spirochete: Insights Into Recombinational Switching With a Suggested Role for Error-Prone Repair

Affiliations
Free article

Antigenic Variation in the Lyme Spirochete: Insights Into Recombinational Switching With a Suggested Role for Error-Prone Repair

Theodore B Verhey et al. Cell Rep. .
Free article

Abstract

The Lyme disease spirochete, Borrelia burgdorferi, uses antigenic variation as a strategy to evade the host's acquired immune response. New variants of surface-localized VlsE are generated efficiently by unidirectional recombination from 15 unexpressed vls cassettes into the vlsE locus. Using algorithms to analyze switching from vlsE sequencing data, we characterize a population of over 45,000 inferred recombination events generated during mouse infection. We present evidence for clustering of these recombination events within the population and along the vlsE gene, a role for the direct repeats flanking the variable region in vlsE, and the importance of sequence homology in determining the location of recombination, despite RecA's dispensability. Finally, we report that non-templated sequence variation is strongly associated with recombinational switching and occurs predominantly at the 5' end of conversion tracts. This likely results from an error-prone repair mechanism operational during recombinational switching that elevates the mutation rate > 5,000-fold in switched regions.

Keywords: Borrelia burgdorferi, Lyme disease; antigenic variation; error-prone repair; expression locus; segmental gene conversion; silent cassettes; vlsE.

Comment in

Similar articles

See all similar articles

Cited by 4 articles

Publication types

MeSH terms

LinkOut - more resources

Feedback