[Localization of mutation leading to resistance of E. coli RNA polymerase to the antibiotic streptolydigin in the gene rpoB coding for the beta-subunit of the enzyme]

Bioorg Khim. 1985 Jan;11(1):132-4.
[Article in Russian]

Abstract

For the first time a mutation of streptolydigin resistance was localized. It was discovered to be a double substitution, namely Gly544----Asp, Phe545----Ser, in the region where most rif-r mutations are located. One may suppose that this region takes part in the formation of both elongation NTP binding site, blocked by streptolydigin, and RNA chain binding and translocation site that is blocked by rifampicin.

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents / pharmacology*
  • Binding Sites
  • Chromosome Mapping
  • DNA Restriction Enzymes
  • DNA-Directed RNA Polymerases / antagonists & inhibitors
  • DNA-Directed RNA Polymerases / genetics*
  • Drug Resistance, Microbial
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Mutation*

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • streptolydigin
  • DNA-Directed RNA Polymerases
  • DNA Restriction Enzymes