Local surface modification at precise position using a chemical pen

Sifeng Mao; Yong Zhang; Qiang Zhang; Jin-Ming Lin; Katsumi Uchiyama

doi:10.1016/j.talanta.2018.05.044

Local surface modification at precise position using a chemical pen

Talanta. 2018 Sep 1:187:246-251. doi: 10.1016/j.talanta.2018.05.044. Epub 2018 May 12.

Authors

Sifeng Mao¹, Yong Zhang², Qiang Zhang¹, Jin-Ming Lin³, Katsumi Uchiyama⁴

Affiliations

¹ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.
² Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji 192-0362, Tokyo.
³ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China. Electronic address: jmlin@mail.tsinghua.edu.cn.
⁴ Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji 192-0362, Tokyo. Electronic address: uchiyama-katsumi@tmu.ac.jp.

PMID: 29853042
DOI: 10.1016/j.talanta.2018.05.044

Abstract

Push-pull cannula system, which was first proposed by Gaddum, has grown to be an important method for the perfusion of brain and region-selective surface treatment. However, reported push-pull cannula systems only concerned on single reagent applications. Microfluidic system was then an exciting tool for multi-reagent treatment on substrate in closed microchannels. Nowadays, it is still a challenge to apply online mixing and reaction for surface pattern in an open environment. Here, we present a novel method using a chemical pen that enables region-selective online chemical reactions for the micro-surface modification/patterning. We utilized this method to fabricate labeling protein array using an online labeling strategy. Moreover, the device was applied for local modification of biomaterials surface by using a three-component reaction at precise position. This tool was the first demonstration of design to perform online reaction of two different reagents on a real solid sample in an open environment. It was demonstrated a useful method for protein array fabrication with online labeled protein.

Keywords: Analytical methods; Bio-interface; Bioreactor; Flow control; Microfluidics.