Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in Macrophages through miR-155

Anal Cell Pathol (Amst). 2018 May 2:2018:5762371. doi: 10.1155/2018/5762371. eCollection 2018.


Aim: To investigate the function of Tremella fuciformis polysaccharides (TFPS) in LPS-induced inflammation and oxidative stress of macrophages.

Methods: RAW264.7 cells were pretreated with TFPS and then stimulated with 0.1 μg/ml LPS. NFκB, Akt, p38MAPK, MCP-1, and SOD-1 were analyzed by Western blotting. Cell viability was measured using MTT assays. Reactive oxygen species (ROS) production, real-time PCR, ELISA, and immunofluorescence staining were performed on RAW264.7 cells that were treated with LPS and/or TFPS to investigate the anti-inflammatory effect of TFPS.

Results: LPS induced inflammation and ROS production and promoted the secretion of cytokines such as TNF-α and IL-6. LPS also enhanced the nuclear translocation of NFκB, which promoted inflammation by oxidative stress. However, pretreatment with TFPS profoundly inhibited the activation of Akt, p38MAPK, and NFκB and attenuated the expression of MCP-1 in macrophages. Meanwhile, TFPS also decreased cytokine and ROS levels and attenuated cell inflammation after treatment with LPS. Moreover, miR-155, one of the key small RNAs which regulate NFκB and inflammation in macrophages, was significantly downregulated.

Conclusion: TFPS inhibits LPS-induced oxidative stress and inflammation by inhibiting miR-155 expression and NFκB activation in macrophages, which suggests that TFPS may be a potential reagent for inhibiting the development of inflammation.

MeSH terms

  • Animals
  • Basidiomycota / chemistry*
  • Cell Death / drug effects
  • Chemokine CCL2 / metabolism
  • Gene Expression Regulation / drug effects
  • Inflammation / drug therapy*
  • Inflammation / genetics
  • Inflammation / pathology
  • Lipopolysaccharides
  • Macrophage Activation / drug effects
  • Macrophage Activation / genetics
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Macrophages / pathology*
  • Mice
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • NF-kappa B / metabolism
  • Oxidative Stress / drug effects*
  • Polysaccharides / pharmacology*
  • Polysaccharides / therapeutic use*
  • RAW 264.7 Cells
  • Signal Transduction / drug effects


  • Ccl2 protein, mouse
  • Chemokine CCL2
  • Lipopolysaccharides
  • MicroRNAs
  • Mirn155 microRNA, mouse
  • NF-kappa B
  • Polysaccharides