A common antigenic motif recognized by naturally occurring human V H 5-51/V L 4-1 anti-tau antibodies with distinct functionalities

Acta Neuropathol Commun. 2018 May 31;6(1):43. doi: 10.1186/s40478-018-0543-z.

Abstract

Misfolding and aggregation of tau protein are closely associated with the onset and progression of Alzheimer's Disease (AD). By interrogating IgG+ memory B cells from asymptomatic donors with tau peptides, we have identified two somatically mutated VH5-51/VL4-1 antibodies. One of these, CBTAU-27.1, binds to the aggregation motif in the R3 repeat domain and blocks the aggregation of tau into paired helical filaments (PHFs) by sequestering monomeric tau. The other, CBTAU-28.1, binds to the N-terminal insert region and inhibits the spreading of tau seeds and mediates the uptake of tau aggregates into microglia by binding PHFs. Crystal structures revealed that the combination of VH5-51 and VL4-1 recognizes a common Pro-Xn-Lys motif driven by germline-encoded hotspot interactions while the specificity and thereby functionality of the antibodies are defined by the CDR3 regions. Affinity improvement led to improvement in functionality, identifying their epitopes as new targets for therapy and prevention of AD.

Keywords: Alzheimer’s disease; Antigenic motif; Monoclonal antibody; Tau protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibody Specificity
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / metabolism*
  • Crystallization
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Immunodominant Epitopes / metabolism
  • Immunoglobulin G / pharmacology*
  • Immunoglobulin Heavy Chains / metabolism*
  • Immunoglobulin Light Chains / metabolism*
  • Male
  • Microglia / metabolism
  • Microscopy, Atomic Force
  • Middle Aged
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Aggregates
  • Young Adult
  • tau Proteins / immunology*
  • tau Proteins / metabolism*

Substances

  • Immunodominant Epitopes
  • Immunoglobulin G
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Protein Aggregates
  • tau Proteins