In vitro-microenvironment directs preconditioning of human chorion derived MSC promoting differentiation of OPC-like cells

Ramesh Periasamy; Daniel V Surbek; Andreina Schoeberlein

doi:10.1016/j.tice.2018.04.002

In vitro-microenvironment directs preconditioning of human chorion derived MSC promoting differentiation of OPC-like cells

Tissue Cell. 2018 Jun:52:65-70. doi: 10.1016/j.tice.2018.04.002. Epub 2018 Apr 7.

Authors

Ramesh Periasamy¹, Daniel V Surbek¹, Andreina Schoeberlein¹

Affiliation

¹ Department of Obstetrics and Gynecology, University Hospital Bern, University of Bern, Switzerland.

PMID: 29857830
DOI: 10.1016/j.tice.2018.04.002

Abstract

The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown either in serum replacement medium (SRM) or serum-containing medium (SM) and tested for their morphology, proliferation, secretome, migration, protein expression and differentiation into OPC-like cells. MSC cultured in SRM condition have distinct morphology/protein expression profile, increased cell proliferation/migration and capacity to differentiate into OPC-like cells.

Keywords: Chorion; Culture conditions; Extracellular matrix; Mesenchymal stem cells; Microenvironment; Neurogenic differentiation; OPC; Placenta; Stem cells.

MeSH terms

Cell Culture Techniques
Cell Differentiation*
Chorion / cytology*
Culture Media, Conditioned
Female
Humans
Mesenchymal Stem Cells / cytology*
Oligodendrocyte Precursor Cells / cytology*
Pregnancy

Substances

Culture Media, Conditioned