[Effect of lupeol on migration and invasion of human breast cancer MDA-MB-231 cells and its mechanism]

Yao Xue Xue Bao. 2016 Apr;51(4):558-62.
[Article in Chinese]


In this study, we examined the inhibitory effects of lupeol, an extract of Euphorbia fischerana Steud, on human breast cancer MDA-MB-231 cells migration and invasion. Lupeol was found to inhibit the invasion of MDA-MB-231 in the cell adhesion assay, transwell test and wound healing assay. The expression of cyclooxygenase-2 (COX-2), matrix metalloproteinase-2 (MMP-2), -9 (MMP-9) and nuclear transcription factor-kappa B (NF-κB) p65 in breast cancer following treatment with different concentrations of lupeol was analyzed with Western blot. Lupeol inhibited the migration and invasion of MDA-MB-231 cells in a dose- dependent manner in vitro (P < 0.05). The expression of COX-2, MMP-2, MMP-9 and NF-κB p65 levels was significantly down-regulated. These observations suggest that lupeol can inhibit the abilities of invasion of MDA-MB-231 cells by inhibiting the protein expression of COX-2, MMP-2 and MMP-9. Its mechanism may be related to inhibition of the nuclear NF-κB signal pathway.

MeSH terms

  • Breast Neoplasms
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Cyclooxygenase 2 / metabolism
  • Down-Regulation
  • Humans
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / metabolism
  • Neoplasm Invasiveness
  • Pentacyclic Triterpenes / pharmacology*
  • Signal Transduction*
  • Transcription Factor RelA / metabolism


  • Pentacyclic Triterpenes
  • RELA protein, human
  • Transcription Factor RelA
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • MMP9 protein, human
  • Matrix Metalloproteinase 9
  • lupeol