MiRNA-seq reveals that miR-124-3p inhibits adipogenic differentiation of the stromal vascular fraction in sheep via targeting C/EBPα

Domest Anim Endocrinol. 2018 Oct:65:17-23. doi: 10.1016/j.domaniend.2018.05.002. Epub 2018 May 9.

Abstract

MicroRNAs (miRNAs) are small noncoding 20-25 nt RNA molecules that regulate gene expression by posttranscriptional repression of messenger RNA. There have been few investigations on the profiles and functions of miRNAs in ovine subcutaneous fat; their roles in the metabolism and deposition of subcutaneous fat also remain unclear. In this study, small RNA libraries were constructed for 2 important Chinese local sheep breeds, Small-tailed Han Sheep, and Shanxi Meat Sheep Dam Line, and used for high-throughput sequencing. Differentially expressed miRNAs were identified, revealing the effect of miR-124-3p on adipogenic differentiation by targeting C/EBPα. Our results provide both a comprehensive understanding of miRNA expression patterns in sheep subcutaneous fat and an insight into the specific roles of miRNAs in adipogenesis.

Keywords: C/EBPα; Sheep; Subcutaneous fat; miR-124-3p; miRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adipogenesis / genetics*
  • Animals
  • Binding Sites
  • Cell Differentiation*
  • Gene Expression
  • Gene Library
  • High-Throughput Nucleotide Sequencing
  • MicroRNAs / analysis
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Sheep / genetics
  • Steroid Isomerases
  • Subcutaneous Fat / blood supply
  • Subcutaneous Fat / chemistry
  • Subcutaneous Fat / metabolism
  • Transfection

Substances

  • 3' Untranslated Regions
  • MicroRNAs
  • Steroid Isomerases
  • EBP protein, human