A New Primer Set to Amplify the Mitochondrial Cytochrome C Oxidase Subunit I (COI) Gene in the DHA-Rich Microalgae, the Genus Aurantiochytrium

Microbes Environ. 2018 Jul 4;33(2):227-229. doi: 10.1264/jsme2.ME17145. Epub 2018 Jun 1.

Abstract

This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

Keywords: Aurantiochytrium; COI; mitochondrial primer.

Publication types

  • Validation Study

MeSH terms

  • DNA, Mitochondrial / genetics*
  • DNA, Protozoan / genetics
  • Electron Transport Complex IV / genetics*
  • Genetic Variation
  • Microalgae / classification
  • Microalgae / enzymology
  • Microalgae / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Stramenopiles / classification
  • Stramenopiles / enzymology
  • Stramenopiles / genetics*

Substances

  • DNA, Mitochondrial
  • DNA, Protozoan
  • Electron Transport Complex IV