Capillary blood microsampling to determine serum biopharmaceutical concentration: Mitra® microsampler vs dried blood spot

Bioanalysis. 2018 Jun 1;10(11):815-823. doi: 10.4155/bio-2018-0010. Epub 2018 Jun 4.


Aim: For assessment of concentrations of biopharmaceuticals, for example, therapeutic drug monitoring, dried blood sampling of capillary blood is a convenient alternative to traditional venepuncture sampling. We investigated an alternative to dried blood spot collection on filter paper: sampling capillary blood using the Mitra® microsampler.

Materials and methods: Therapeutic monoclonal antibodies were spiked in whole blood, sampled using filter paper and Mitra microsampler and concentrations measured using specific ELISAs.

Results: Good recoveries of adalimumab, infliximab, ustekinumab, vedolizumab, tocilizumab, natalizumab and rituximab were found up to 1 month of storage at room temperature, averaging 95.2% for the Mitra microsampler and 92.9% for Whatman® paper. Both hemoglobin and potassium yield satisfactory estimates for the volume of the cellular fraction of blood samples in combination with the Mitra microsampler.

Conclusion: We established practical protocols for the estimation of serum/plasma concentrations of therapeutic antibodies via capillary blood microsampling.

Keywords: DBS; biologic; capillary blood microsampling; dried blood spot; therapeutic monoclonal antibody.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal / blood*
  • Dried Blood Spot Testing / methods*
  • Drug Monitoring
  • Humans
  • Microtechnology / methods*
  • Time Factors


  • Antibodies, Monoclonal