MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF

Biochem Biophys Res Commun. 1985 Apr 30;128(2):898-905. doi: 10.1016/0006-291x(85)90131-7.


Epidermal growth factor (EGF) has been noted to stimulate proliferation of a variety of normal and malignant cells including those of human breast epithelium. We report here that MDA-468, a human breast cancer cell line with a very high number of EGF receptors, is growth-inhibited at EGF concentrations that stimulate most other cells. The basis for the elevated receptor level is EGF receptor gene amplification and over-expression. An MDA-468 clone selected for resistance to EGF-induced growth inhibition shows a number of receptors within the normal range. The results are discussed in relation to a threshold model for EGF-induced growth inhibition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / genetics*
  • Cell Division / drug effects
  • Cell Line
  • DNA / analysis
  • DNA Restriction Enzymes / metabolism
  • Deoxyribonuclease HindIII
  • Epidermal Growth Factor / pharmacology*
  • ErbB Receptors
  • Gene Amplification*
  • Humans
  • Receptors, Cell Surface / genetics*


  • Receptors, Cell Surface
  • Epidermal Growth Factor
  • DNA
  • ErbB Receptors
  • DNA Restriction Enzymes
  • Deoxyribonuclease HindIII