Molecular characterization of lumpy skin disease virus in Namibia, 2017

Arch Virol. 2018 Sep;163(9):2525-2529. doi: 10.1007/s00705-018-3891-x. Epub 2018 Jun 4.

Abstract

Between January and July 2017, lumpy skin disease (LSD) outbreaks were reported in cattle in Namibia. DNA was extracted from skin biopsies taken from 32 cattle, and the RNA polymerase 30 kDa subunit (RPO30) gene of the LSD virus (LSDV) was successfully amplified by PCR. Phylogenetic analysis revealed that the newly sequenced LSDV isolates from Namibia were identical to LSDV isolates identified previously in Burkina Faso, Egypt, Greece, Niger, Serbia and South Africa. Given that only unvaccinated herds were affected by LSD, it is recommended that the current vaccination programmes in Namibia be re-evaluated to allow nationwide coverage.

MeSH terms

  • Animals
  • Cattle
  • DNA, Viral / genetics*
  • DNA-Directed RNA Polymerases / genetics*
  • Disease Outbreaks*
  • Immunization Programs / organization & administration
  • Lumpy Skin Disease / epidemiology*
  • Lumpy Skin Disease / pathology
  • Lumpy Skin Disease / virology
  • Lumpy skin disease virus / classification
  • Lumpy skin disease virus / genetics*
  • Lumpy skin disease virus / isolation & purification
  • Namibia / epidemiology
  • Phylogeny
  • Protein Subunits / genetics
  • Viral Proteins / genetics*

Substances

  • DNA, Viral
  • Protein Subunits
  • Viral Proteins
  • DNA-Directed RNA Polymerases